Preliminary Programme

 

1^st Day:  Basics of Ultramicrotomy

Morning: Lectures
Basics of ultramicrotomy including ultra-sonic diamond knife Embedding & Staining methods of polymers & biological preparation Discussion and procedure of the samples to be examined

Afternoon: Practical work
Specimen preparation, hands-on Ultramicrotomy (room temperature)

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2^nd Day:  Cryo-Ultramicrotomy

Morning: Lectures
Specimen preparation; Challenging samples Cryo-TEM Background overview in CEMOVIS, High Pressure Freezing and CLEM

Afternoon: Practical work
Hands-on (Cryo-)Ultramicrotomy; Getting contrast

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3^rd Day:  Applications & Analysis

Morning: Lectures
In-situ microscopy; Image processing; Particle analysis; 3D Ultramicrotomy (AtumTome, Artos)

Afternoon: Practical work
Hands-on (as required); Analysis of specimens (TEM, SEM)

Faculty during the lectures and practical work:

• FELMI-ZFE, Graz: Our Team – Your Solution Provider
• Diatome, Biel: https://diatome.ch/
• Leica Microsystems, Vienna: https://www.leica-microsystems.com/
• Science Services, Munich: https://www.scienceservices.de/